https://June6699.github.io/tags/%E7%94%9F%E4%BF%A1/%E6%96%87%E7%AB%A0/Recent content in 生信/文章 on June's BlogHugozh-cnTue, 10 Mar 2026 00:00:00 +0000https://June6699.github.io/posts/article2_limca_hic+rna_atac/Tue, 10 Mar 2026 00:00:00 +0000https://June6699.github.io/posts/article2_limca_hic+rna_atac/<h3 id="--limca--nature-methods-2024">📌 ② LiMCA — <em>Nature Methods</em>, 2024</h3> <p><strong>Wu H, Zhang J, Jian F, et al. “Simultaneous single-cell three-dimensional genome and gene expression profiling uncovers dynamic enhancer connectivity underlying olfactory receptor choice.” <em>Nat Methods</em> 21, 974–982 (2024)</strong></p> <p><a href="https://www.nature.com/articles/s41592-024-02239-0">https://www.nature.com/articles/s41592-024-02239-0</a></p> <p><strong>亮点：</strong> LiMCA（Linking mRNA to Chromatin Architecture）通过物理分离同一细胞的细胞质（mRNA）和细胞核（染色质），实现了对 3D 基因组结构和转录组的高灵敏度联合检测。研究者将 LiMCA 与他们开发的高分辨率 scATAC-seq 方法（METATAC）结合，成功解析了单个嗅觉感觉神经元发育过程中的染色质可及性、3D 基因组结构和基因表达信息，扩展了已知的嗅觉受体增强子库，揭示了“一个神经元—一个受体”选择过程背后动态的空间调控规律。</p>https://June6699.github.io/posts/article1-hires-hic+rna-seq%E5%85%B3%E8%81%94/Thu, 05 Mar 2026 00:00:00 +0000https://June6699.github.io/posts/article1-hires-hic+rna-seq%E5%85%B3%E8%81%94/<h2 id="--hires--science-2023">📌 ① HiRES — <em>Science</em>, 2023</h2> <p><a href="https://www.science.org/doi/10.1126/science.adg3797">https://www.science.org/doi/10.1126/science.adg3797</a></p> <p><strong>Liu Z, Chen Y, Xia Q, et al. &ldquo;Linking genome structures to functions by simultaneous single-cell Hi-C and RNA-seq.&rdquo; *Science* 380, 1070–1076 (2023)</strong></p> <p><strong>亮点：</strong> 这是首篇真正实现单细胞水平同时测量染色质三维构象和基因表达的方法论文。作者开发了 HiRES（Hi-C and RNA-seq Employed Simultaneously）技术，并应用于数千个小鼠发育胚胎单细胞。研究发现，<strong>三维基因组结构在高度受细胞周期影响的同时，会随发育进程以细胞类型特异性方式逐渐分化</strong>。通过比较染色质互作与转录的拟时序动态，他们发现了<code>广泛的&quot;染色质重塑先于转录激活&quot;现象</code>，证明特异性染色质互作与谱系分化过程中的转录调控密切相关。</p>